Depending on your needs, the length of the recombination reaction can be extended up to 20 hours. Gateway technology is schematically represented below. Invitrogen gateway lr clonase ii enzyme mix is a proprietary enzyme and buffer formulation. If youre having trouble viewing any pdf files, you may need to install adobe reader on your computer, tablet or smartphone. Locate drivers, user guides and product specifications for your gateway technology. Gateway cloning is rapid, robust, and highly amenable to highthroughput. It is a molecular biology method that enables researchers to efficiently transfer dna fragments between plasmids using an appropriate set of recombination sequences, the gateway att sites, and two proprietary enzyme mixes, called lr clonase, and bp clonase. Gateway cloning technology instruction manual, but does not provide rights to. Gateway bp clonase ii contains enzymes and buffer in a single mix to enable convenient tenmicroliter reaction set up with fewer pipetting steps. Gateway technology is a universal cloning method that takes advantage of the site. Since gateway cloning uses patented recombination sequences, and proprietary enzyme mixes available only from invitrogen, the technology does not allow researchers to switch vendors and contributes to the lockin effect of all such patented procedures.
Transfer gene from entry clone into destination vector with lr clonase enzyme. An overnight incubation typically yields 5 times more colonies than a 1hour. Gateway bp clonase ii enzyme mix life technologies bp and lr clonase facilitate the recombination for more detail, refer to the gateway manual. The full power of gateway cloning is realized with multisite gateway pro technology, which allows for the simultaneous assembly of multiple fragments into a single vector in a predefined order, orientation, and reading frame figures 5 and 6. Schematic modified from the invitrogen multisite gateway pro user manual. Bp and lr recombination reaction protocols for experienced users. Conventions used in this guide this manual uses the following documentation conventions. An example of using multisite gateway pro technology to study expression of multiple genes. A10462, a10463, a10464, a10465, a10467 version a 6 jun 2008. Documentation conventions description represents examples arial font. Invitrogen gateway recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system in just a few simple steps. Gateway cloning principle and applications and advantages.
The consideration paid for this product grants a limited license with a paid up royalty to use the product pursuant to the terms set forth in the accompanying limited label license. Several invitrogen pentr vectors are available for directional topo. A complete user manual is provided as supplemental material. Recombinational cloning using gateway and infusion. This product is covered by a limited label license see section 1. Cloning methods gateway one tube protocol to create a. Find the office and computer equipment manual you need at manualsonline. Page 1 specifications for gateway solo 5100 multimedia notebook specifications are subject to change without notice or obligation. With the appropriate entry and destination vectors, one can use gateway to clone a gene of interest into a variety of expression systems. The lr clonase ii mix is quite rich in glycerol so proper mixing is essential. The gateway cloning system, invented and commercialized by invitrogen life technologies since the late 1990s. Gateway lr clonase ii contains enzymes and buffer in a single mix to enable convenient tenmicroliter reaction setup with fewer pipetting steps. If you received this modem for an nbn service from 2019 onwards, simply follow the plugin steps and your modem will configure automatically. Gateway lr clonase ii enzyme mix thermo fisher scientific.
Gateway cloning gives researchers the opportunity to easily transfer dna fragments into plasmids using proprietary recombination sites called gateway att sites and either the lr clonase or bp clonase enzyme mixes gateway cloning is efficient, but it still has multiple steps that can be optimized. By checking this box, i agree to receive marketing communications and product offers by email from fluke electronics corporation, transacting under fluke industrial, or. Gateway bp clonase ii contains enzymes and buffer in a single mix to enable. Submit a question directly to technical support email protected search. Through a twostep process, any gene or dna fragment can easily be cloned in a directional and reversible manner into an entry vector before transferring into a destination vector for expression. The steve clough lab university of illinois department. The donor vector box, the gateway bp clonase ii enzyme mix and reagents box.
Please see page of the gateway technology with clonase ii manual for the what is the difference between bp clonase and bp. Gateway bp clonase ii enzyme mix catalyzes the in vitro recombination of pcr. This manual provides an overview of the gateway technology and provides. User manual corporate headquarters invitrogen corporation 1600 faraday avenue carlsbad, ca 92008.
Three way gateway reactions for modular gene targeting. The internet archive manual library is a collection of manuals, instructions, walkthroughs and datasheets for a massive spectrum of items. It is the end user s responsibility to consult the applicable msdss before using this product. An advance in recombination cloning technology was the development of multifragment recombination cloning that allows simultaneous cloning of more than one dna. Since its invention in the late 1990s, gateway cloning technology has become very popular as a rapid and highly efficient way to move dna sequences into multiple vector systems. Manuals covering electronic and mechanical products, instructions on mixing or blending items, and instruction sets for software and computer items are all included. Gateway bp clonase ii enzyme mix catalyzes the in vitro recombination of pcr products or subcloning dna segments from clones containing attb sites and a donor vector containing attp sites to generate entry clones.
To locate your free gateway manual, choose a product type below. Gateway destination vectors are commercially available from invitrogen, emd and covalys. Disposal of waste organics, acids, bases, and radioactive materials must comply with all. Not intended for any animal or human therapeutic or diagnostic use.
User manual corporate headquarters invitrogen corporation 1600 faraday avenue carlsbad, ca 92008 t. The gateway cloning system introduction to the gateway system contents defining gateway technology. Gateway bp clonase ii enzyme mix thermo fisher scientific. Three way gateway reactions for modular gene targeting vector assembly. Find the user manual and the help you need for the products you own at manualsonline. Select your model number to find the installation manual for your fireplace. A free powerpoint ppt presentation displayed as a flash slide show on id. Invitrogen gateway cloning technology has been cited. Gateway lr clonase ii enzyme mix product information sheet. Gateway cloning scienceftw wiki fandom powered by wikia. Yeast twohybrid system combined with the gateway technology will greatly. Find printable instructions to assist with selfinstallation for the following cox services and devices. Mix 12 ml of lr reaction mix with 50 ml competent dh5a cells for the preparation see the appropriate protocol. Gateway technology with clonase ii thermo fisher scientific.
860 676 338 379 360 1448 871 1434 496 701 554 533 1006 765 551 1019 373 706 652 873 295 1396 1172 96 1089 543 294 565 592 33 321 799 1300 1382 736 1425 1088 664 1118 1318